Lisboa (Portugal) 23 – 27 July 2018


Limited registrations16 participants

Learn about the latest developments on Epithelial Systems: Physiology and Pathophysiology and discuss your project with the experts

Workshop Overview

Participants will learn about the latest developments in Epithelial Systems: Physiology and Pathophysiology. Besides the opportunity to discuss their projects with the experts, participants will be able to acquire practical skills on:

  • production, cultivation and characterization of epithelial cells
  • collection of nasal cells
  • cultivation of intestinal organoids
  • functional approaches to assess CFTR

Who should apply?
The workshop is directed at PhD Students, Post-Docs, Technicians and Independent Researchers.
Participants are expected to be acquainted with the basics on Cystic Fibrosis field.

The course is promoted by BioISI, as an initiative of the ECFS Basic Science Working Group.

Course organizers
Margarida Amaral (BioISI / FCUL)

Course Secretariat
Simão Luz (BioISI / FCUL)

Venue  The workshop will take place at Faculty of Sciences of the University of Lisbon (FCUL), in Portugal. Lectures and seminars will be held at C1 campus building, and practical tutorials will be held at the C8.

Click on the photo for map directions


Fee: Free Registration (sponsored by ECFS)

Includes course materials and coffee breaks.

(Participants should arrange their own travel and accommodation. However, a few travel grants might be available. Check for more information below.)

For additional Information:

The Netherlands

2 travel grants supported by the Dutch Cystic Fibrosis Foundation (NCFS) for Dutch participants.


Travel Grants supported by the German CF patient organization, available for participants from Germany.


2 travel grants supported by the Belgian patient organization for participants from Belguium (400€ each)

Lecture Topics

  • Cell Culture of Epithelial Cells, Tissues and Organoids
  • Polarized Cells: Characteristics & Pathways
  • Cystic Fibrosis: a Disease of Multilpe Epithelial Tissues
  • Physiology of Airway Surface Liquid
  • Organoids as Model Systems to Epithelia
  • Physiology of the Airway Epithelial Cells
  • Physiology of the Intestinal Epithelial Cells
  • Physiology of the Pancreas and Sweat Gland Electrophysiology techniques

Lab work and Tutorials

  • Culture of Primary Human Nasal Cells
  • Airway Surface Liquid Microscopy Measurements
  • Culture of Intestinal Organoids
  • Immunofluorescence of Epithelial Cells & Tissues
  • Differentiation & Regeneration Assays
  • Organoids Swelling Assay
  • Ussing Chamber Analysis of Native Tissues and Polarized Epithelial Cells

Meet the Expert Sessions

Instructors will be available for informal discussions during the course with participants


Find provisional schedule here.