The Facility provides an integrated environment for research projects which interrogate large compound or nucleic acid libraries or require agile or robotized sample handling. Facility staff provides support at all stages of a screening campaign from assay design to sample preparation, instrument operation, bioimage quantification, data analysis and software development. The Facility also offers advanced training events on microscopy, high content screening and bioimage analysis.
A set of sample preparation technologies enable efficient handling of cell-based assays and library management. Once data is acquired, short-term local storage is offered for screening datasets.
HIGH-CONTENT AND HIGH-THROUGHPUT SCREENING
The Facility hosts screening projects based on microscopy or plate reader readouts. Image-based assays provide rich phenotypic data for individual cells (high content). Our imaging systems are capable of live cell assays and multimodal imaging. High-throughput plate reader measurements (absorption, fluorescence, luminescence) enable efficient screening projects which can be combined with fast online reagent injection.
Microscopy systems provide imaging capabilities for screening or non-screening projects. Most systems are fully motorized and compatible with live specimens.
Available imaging modalities:
- Brightfield Microscopy (phase contrast, DIC)
- Widefield fluorescence
- Confocal fluorescence
- Fluorescence Lifetime Imaging (FLIM)
- Förster Resonance Energy Transfer imaging (FRET)
- High-throughput microscopy
- Live cell imaging
The Facility provides support on bioimage analysis, with a focus on large imaging datasets. Our users have the oportunity to be trained on the tools which better solve their biological requirements. We have a track record on the development of assay-specific bioimage analysis pipelines, exploratory data analysis software and statistical data analysis.