FCUL Microscopy Facility (FCUL-MF) is a research and training infrastructure for microscopy and bioimaging. FCUL-MF is also a node of the Portuguese Platform for Bioimage.

FCUL-MF functions as a service provider and technical support hub on:

  • Stereo, widefield fluorescence,confocal and electron microscopy
  • FRET/FLIM and High throughput microscopy
  • Image analysisand quantification techniques
  • Hands-on courses in basic and advanced microscopy techniques and image analysis


Rui Malhó (rmmalho@fc.ul.pt)

Hugo Botelho (hmbotelho@fc.ul.pt)


The High-Throughput Screening Facility is a research infrastructure for spectroscopy and image-based screening. The facility provides specialized support and open access to BioISI, Academy and Industry users for research projects on Drug Discovery, Functional genomics and Bioimaging. Our key specialization is High-Content Screening.

Services include: sample preparation, high-content and high-throughput screening, bioimaging (automated widefield, confocal fluorescence and FLIM) and bioimage analysis for drug screening, functional genomics, cell phenotyping and others.

Equipment: Leica TCS SP8 Falcon, Leica DMI6000B, PerkinElmer Janus, PerkinElmer ictor 3V, Tecan Infinite F200 Pro, Tecan Hydrospeed, ThermoFisher Multidrop combi, Mettler Toledo Liquidator 96, GeneVac MiVac Duo



Hugo Botelho (hmbotelho@fc.ul.pt)


The BioISI Genomics Facility is a research facility and service provider of high-quality omics data from multiple biological sources based on biomolecular nanopore sensing technologies. The aim is to support and consolidate the concept of Biology 4.0 and to empower the scientific community in the development path towards the fields of Digitization of Life and Synthetic Biology.

Services include: whole/targeted genome sequencing, metagenomics analysis, – Certification of cellular lines and reference strains, Native genomic DNA Sequencing (without amplification), Native DNA Methylation profiling (6mA; 5mC).

Equipment: Native realtime DNA/RNA Sequencing (Throughput 25 Gbp per day);

GridION X5; Minion; Digital Microfluidics Station for automate sample preparation.




The BioISI Physics Facility is a research facility and service provider dedicated to the exploration of the Nanoworld, where the potential of the Atomic Force Microscope (AFM) for probing biological interactions plays a central role.

Services include: measuring and analysing data from AFM related techniques for biological samples, analysis of topographic images in tapping and contact modes, force curve analysis using our own home-made software for mechanical properties studies.

Equipment: conventional AFM, custom-made optical-fiber based FFM (Viriato), custom-made conventional AFM.




The BioISI Mass Facility is a research infrastructure dedicated to analyse and identify compounds by liquid chromatography coupled with mass spectrometry.

Services include the identification of small molecules, cell metabolites and proteins and separation and identification of biomolecules using an Elute UHPLC coupled with Impact II (QqTOF), Brucker system.


Helena Gaspar (Bioisimass@fc.ul.pt)